Reaction of Ehrlich ascites cells in exposure to oxygen at high pressure.

Suspensions of Ehrlich ascites mouse tumor cells were exposed in vitro to 02 at 75-80 pounds/sq in guage for periods of from 12 to 24 hr. Control suspensions were exposed to air at atmos pheric pressure, O2 at atmospheric pressure, and air at high pres sure. It was found that 02 at high pressure has a deleterious action on these cells, as manifested by: increased numbers of damaged cells in the suspensions as determined by Trypan blue; morphologic alterations as revealed by phase microscope char acterized by large, usually eccentric, "halos" or blebs, for which